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. 2017 Jun 30;7:4418. doi: 10.1038/s41598-017-02664-6

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Histogram of filament length or flagella number in Salmonella strains. (a,b) Each strain with or without pPint-FliD, pPint-FliD (L443R), pPlac-FliD, pPlac-FliT, pPlac-EGFP-FliD-FLAG, or pPlac-FliD-FLAG was grown in LB medium to mid-log phase, and filament length or flagella number was measured. pPint-FliD and pPint-FliD (L443R) are pACYC184 derivatives expressing fliD or fliD (L443R) under the intrinsic promoter, while pPlac-FliD, pPlac-FliT, pPlac-EGFP-FliD-FLAG, and pPlac-FliD-FLAG are pUHE21-2lacI ^q derivatives overexpressing fliD, fliT, EGFP-fliD-FLAG, or fliD-FLAG, respectively, under the lac promoter.