Table 2.
Change in solvent accessible surface area (SASA, Å2) of glycans on monomer 1 due to the omission of selected glycans for which the change in SASA was >5 Å. Average values and standard errors (SE) are shown.
| Glycan omitted | Glycan | Average difference | SE | Glycan omitted | Glycan | Average difference | SE |
|---|---|---|---|---|---|---|---|
| g88 | g618 | 10.4 | 4.3 | g363 | g133 | 9.6 | 4.3 |
| g88 | g625 | 56.3 | 8.6 | g363 | g386 | 202.4 | 10.1 |
| g133 | g197 | 33.1 | 7.8 | g363 | g392 | 105.6 | 9.6 |
| g133 | g363 | 11.9 | 5.5 | g363 | g398 | 20.8 | 8.8 |
| g133 | g386 | 68.6 | 12.0 | g386 | g133 | 70.8 | 12.4 |
| g137 | g156 | 31.4 | 9.1 | g386 | g332 | 16.6 | 6.6 |
| g137 | g301 | 36.5 | 11.6 | g386 | g363 | 211.9 | 9.3 |
| g137 | g332 | 26.9 | 9.3 | g386 | g392 | 87.0 | 14.0 |
| g156 | g137 | 31.1 | 9.1 | g392 | g332 | 47.9 | 12.7 |
| g197 | g133 | 31.9 | 7.5 | g392 | g363 | 108.2 | 10.1 |
| g234 | g276 | 111.3 | 12.3 | g392 | g386 | 93.3 | 15.6 |
| g234 | g637 | 33.9 | 7.6 | g392 | g398 | 52.1 | 12.5 |
| g262 | g295 | 9.4 | 3.0 | g392 | g406 | 8.8 | 4.5 |
| g262 | g301 | 19.2 | 5.5 | g398 | g355 | 10.2 | 5.9 |
| g262 | g448 | 42.7 | 3.8 | g398 | g363 | 19.3 | 8.0 |
| g276 | g234 | 99.0 | 10.9 | g398 | g392 | 48.2 | 11.5 |
| g276 | g637 | 22.6 | 7.5 | g398 | g406 | 13.9 | 5.7 |
| g295 | g262 | 10.7 | 3.7 | g406 | g332 | 7.5 | 3.1 |
| g295 | g301 | 6.1 | 5.3 | g406 | g355 | 26.3 | 9.7 |
| g295 | g332 | 14.6 | 4.3 | g406 | g392 | 9.5 | 4.4 |
| g295 | g448 | 106.5 | 14.5 | g406 | g398 | 16.5 | 6.5 |
| g301 | g137 | 40.2 | 13.1 | g406 | g448 | 28.8 | 12.3 |
| g301 | g262 | 17.5 | 5.0 | g448 | g262 | 36.5 | 3.5 |
| g301 | g295 | 6.8 | 6.0 | g448 | g295 | 103.1 | 14.3 |
| g332 | g137 | 31.4 | 9.5 | g448 | g406 | 27.5 | 11.9 |
| g332 | g295 | 15.1 | 4.3 | g611 | g637 | 5.0 | 5.0 |
| g332 | g386 | 16.5 | 6.5 | g618 | g88 | 11.3 | 5.0 |
| g332 | g392 | 46.4 | 12.2 | g618 | g625 | 40.2 | 10.6 |
| g332 | g406 | 8.5 | 3.6 | g625 | g88 | 56.7 | 8.7 |
| g355 | g398 | 9.3 | 5.5 | g625 | g618 | 34.4 | 9.0 |
| g355 | g406 | 29.4 | 10.6 | g637 | g234 | 33.4 | 7.3 |
| g637 | g276 | 25.3 | 8.4 |
Calculation of the change in SASA or AASA was performed by first determining the overall SASA or AASA for all atoms in the Env followed by determination of the SASA or AASA contribution of the individual glycans or protein residues, respectively. This procedure was then repeated with the overall SASA or AASA calculation performed with the individual, selected glycan omitted. The difference between the SASA or AASA values of the glycans or protein residues, respectively, between the truncated and full Env were then determined.