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. 2017 May 3;33(4):373–388. doi: 10.1007/s10565-017-9394-9

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© The Author(s) 2017

Open Access This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.

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Fig. 2 — Induction of circadian rhythm in serum-shocked human CCD-34Lu fibroblasts. a Expression of PER1, PER2, and BMAL1 transcripts obtained using qRT-PCR analysis at the indicated time points beginning at serum shock (0 h). The values are normalized with GAPDH mRNA as an internal control and plotted as percentages (means ± SD). b Representative western blot of PER2 protein at the same time points after serum shock and its quantification expressed in the fold-change (FC) relative to GAPDH protein as loading control (c)