Figure 2.

Time course analyses of amPER immunoreactivity in whole brains. Forager, nurse-age and nurse bees were sampled over 1.5 days in constant darkness and amPER brain expression analysed by WBs (n = 5 pooled brains per time point, 1 time course each). The band representing full-length amPER (a) oscillated in intensity relative to the loading control actin (c), the quantification of which is displayed in (b). Photoperiod prior to DD is indicated in black and grey bars above the graph. The term ‘nurse-age’ refers to bees of 7–9 days of age that were removed from the brood comb several hours before the onset of sampling.