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. 2017 Jun 15;8:111–122. doi: 10.1016/j.omtn.2017.06.008

Figure 2.

Figure 2

miR-135b Promotes Stem Cell-like Properties in OS Cells

(A) miR-135b was overexpressed in CD133-positive and ALDH1-positive OS cells compared with CD133-negative and ALDH1-negative OS cells. (B) CD133-positive populations were increased in HuO9 and Saos-2 cells that stably expressed miR-135b compared with their corresponding vector control cells. (C) ALDH1-positive populations were increased in HuO9 and Saos-2 cells that stably expressed miR-135b compared with their corresponding vector control cells. (D) The expression of cancer stem cell marker proteins was dramatically increased in cells that stably expressed miR-135b compared with the control. (E) Overexpression of miR-135b stimulated osteosphere formation in the indicated cell lines. *p < 0.05; **p < 0.01.