Figure 6.

Clinical Relevance of miR-135b Expression and the Expression of Its Target Genes in Human OS Tissues

(A) The correlation between the miR-135b level and the 5-year overall survival and the recurrence-free survival of 112 patients with OS was analyzed using the Kaplan-Meier method. (B) The correlation between miR-135b and its target genes in OS specimens was determined by qRT-PCR and immunohistochemical analysis, which were used to measure the expression of miR-135b and the expression of its target genes, respectively. (C) The correlation between the Notch1 and β-catenin levels with the 5-year overall survival rate in 112 patients with OS was analyzed using the Kaplan-Meier method. (D) Illustration of Notch and Wnt/β-catenin activation by miR-135b via the suppression of multiple target genes.