Fig. 5.

Role of Nrf2 in the neuronal differentiation of NSPCs. A, DCX and DAPI staining of neurospheres from 3-months old mice grown under differentiation conditions. B, measurement of the cell migration as determined by distance from the neurosphere edge (dotted line) to DAPI stained nuclei (n = 40). C, quantification of DCX⁺ cells from neurospheres shown in A (n = 10). D, sholl analysis of the neurons in the differentiated neurospheres derived from 3-months old mice (n = 12). E. DCX and DAPI staining of Nrf2^-/- neurospheres rescued by lentiviral expression of NRF2^ΔETGE. F, measurement of their cell migration distance (n = 40). G, quantification of DCX⁺ cells (n = 10). H-J, parallel analysis in Nrf2^+/+ neurospheres infected with control of shRNA lentivirus to knockdown NRF2. Data represent mean values ± SEM. *p < 0.05, **p < 0.01 and ***p < 0.001 according to a Student's t-test vs. the Nrf2^-/- group (B, C and D) or the experimental groups (F, G, I and J).