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. 2005 Feb 8;33(3):838–845. doi: 10.1093/nar/gki212

Figure 2.

Figure 2

Pol III dependent transcription from different promoters. Sensitivity of transcription to the aptamers before (A) and after (B) PIC formation. The procedure is schematically represented at the top. The aptamers used are listed on the top of the corresponding lanes. The genes assayed are denoted to the left of the gel. (C) Rescuing aptamer inhibition of Pol III dependent transcription by excess TBP. In each reaction, 2 nM tRNA LEU3 template was incubated with 20 μg yeast whole-cell extract (WCE) for 30 min to allow PIC formation. The transcription reaction was started by the addition of NTPs and allowed to proceed for 30 min. Where indicated, 30 ng of different recombinant proteins, TBP, BSA or TFIIB (about 50 nM for TBP), were mixed with WCE before mixing with the DNA template; aptamer #12 at final concentration of 20 nM was mixed with the DNA template before incubating with WCE.