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. 2005 Feb 8;33(3):838–845. doi: 10.1093/nar/gki212

Figure 3.

Figure 3

TFIIIB•DNA interactions perturbed by aptamers. In the binding reaction, a DNA probe containing the TATA box and its flanking region of the yeast SNR6 gene was radiolabeled by kinase and used to form the TFIIIB•DNA complex (Bdp1•Brf1•TBP•TATA complex). (A) Assembly of the TFIIIB•DNA complex. The components of the various complexes are given to the left of the gel. (B) Prevention of the formation of the TFIIIB•DNA complex by aptamers present during assembly. In the binding reaction, 320 nM of the aptamers or TATA containing DNA oligos were included. (C) Resistance of the preformed TFIIIB•DNA complex to aptamers. The TFIIIB•DNA complex was formed as in (A), then a fraction of the mixture was taken out and mixed with 320 nM of different aptamers or TATA-DNA and incubated for 30 min at room temperature before being loaded onto a native gel.