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. Author manuscript; available in PMC: 2018 Jun 28.
Published in final edited form as: Thromb Haemost. 2017 Apr 13;117(7):1358–1369. doi: 10.1160/TH17-01-0043

Figure 2.

Figure 2

Assessment of thrombin generation in the absence and presence of sTM in normal plasma and plasmas derived from the proband and her younger brother. Citrated normal or test plasmas (80 μL) were incubated with 20 μL PPP reagent and the reaction was initiated with 5 pM TF in the absence or presence of 5 nM or 10 nM sTM. Each sample and the related calibrator were tested in duplicate. The kinetics of thrombin generation was monitored by measuring the hydrolysis of a fluorogenic thrombin substrate. The lag time (LT, min), peak height (Peak, nM), and endogenous thrombin potential (ETP, nM*min) were deduced from thrombin generation curves as described in “Materials and methods”.