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. 2005 Feb 8;33(3):893–902. doi: 10.1093/nar/gki234

Figure 1.

Figure 1

Yeast three-hybrid analysis of TEP1 deletions and vault/telomerase RNAs. (A) TEP1 truncations were made using amino acids 1–871 as a starting point, since this region of human TEP1 interacts with human vault and telomerase RNAs in the yeast three-hybrid system (16). (B) Yeast strain L40i was transformed with plasmids expressing the above TEP1 deletions and murine vault or telomerase RNA in either sense (s) or antisense (as) orientations. Yeast were grown on synthetic drop-out plates lacking uracil, leucine and histidine, and containing 5 mM 3-aminotriazole (+3AT) or synthetic media lacking only uracil and leucine (−3AT). Growth on +3AT media indicates a TEP1–RNA interaction. No TEP1 deletions interacted with antisense RNA constructs (data not shown), but this is shown above only for those TEP1 deletions that interact with RNA in the sense orientation (constructs 1 and 2).