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. 2005 Feb 8;33(3):893–902. doi: 10.1093/nar/gki234

Figure 4.

Figure 4

The mVR point mutant competes with TR for binding to TEP1–RBD. EMSA of mVR double point mutant incubated with 0, 5, 20 and 80 ng of TEP1–RBD (lanes 1–4). Unlabeled mVR mutant or mouse TR (50×) competes with the labeled probe (lanes 5 and 6). Three irrelevant RNAs do not compete with TR for binding to TEP1–RBD (lanes 7–9), but the pBluescript transcript does compete efficiently (lane 10) as in Figure 2. Labeled RNA is indicated with a black dot and shifted complexes are indicated with an asterisk.