Table 1.
Stepwise reversion of ΨmVR to mVR sequence
| Nucleotide | ΨmVR | 1 | 2 | 3 | 4 | 5 | 6 | mVR |
|---|---|---|---|---|---|---|---|---|
| 16 | G | G | G | C | C | C | C | C |
| 28 | U | U | C | C | C | C | C | C |
| 36 | U | U | G | G | G | G | G | G |
| 70 | A | A | A | A | A | A | G | G |
| 73 | U | U | U | U | U | C | U | C |
| 91 | G | G | G | G | A | A | A | A |
| 100 | A | A | A | A | G | G | G | G |
| 121 | G | C | C | C | C | C | C | C |
| Binding | + | + | + | + | + | ± | ± | − |
The position of the 8 nt differences between the two RNAs are shown in the leftmost column, and the base found in ΨmVR and mVR at each is indicated. Columns 1–6 are variant VRs tested for binding to TEP1–RBD, with the result shown in the bottom row.