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Experimental range and level coded of independent variables
| Factors | Symbols | Actual levels of coded factors | ||
|---|---|---|---|---|
| −1 (Low) | 0 (Middle) | +1 (High) | ||
| Time of incubation (days) | A | 11 | 12 | 13 |
| pH | B | 7 | 8 | 9 |
| Temperature (°C) | C | 25 | 30 | 35 |
| Conc of starch (% w/v) | D | 0.5 | 1.0 | 1.5 |
| Conc of tryptone (% w/v) | E | 0.5 | 1 | 1.5 |
