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DPAGE analysis of temperature-dependent photo-crosslinking in the absence and presence of Mg2+ (10 mM). Bands representing new crosslinks are marked accordingly, except G11 which was only observed under the larger-scale conditions of preparatory experiments. The bands marked A–A represent intradomain crosslinks; those marked A–B represent interdomain crosslinks. ‘rz’ indicates uncrosslinked hairpin ribozyme. Temperature units are °C.
