Table 1.
Gene | Primers | Nucleotide sequences 5′-3′ |
---|---|---|
TGF-β1 | Forward | 5′-CGCAACAACGCAATCTATG-3′ |
Reverse | 5′-ACCAAGGTAACGCCAGGA-3′ | |
Smad2 | Forward | 5′-ACTATACCCACTCCATTCCA-3′ |
Reverse | 5′-CACTATCACTTAGGCACTCG-3′ | |
Smad3 | Forward | 5′-CCAGTGCTACCTCCAGTGTT-3′ |
Reverse | 5′-CTGGTGGTCGCTAGTTTCTC-3′ | |
Smad7 | Forward | 5′-GGCTTTCAGATTCCCAACTTC-3′ |
Reverse | 5′-GGCTTTCAGATTCCCAACTTC-3′ | |
GAPDH | Forward | 5′-ATGATTCTACCCACGGCAAG-3′ |
Reverse | 5′-CTGGAAGATGGTGATGGGTT-3′ |