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. 2005 Feb 17;33(3):977–986. doi: 10.1093/nar/gki241

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© The Author 2005. Published by Oxford University Press. All rights reserved

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Concerted integration reaction catalyzed by yeast recombinant HIV-1 IN. (A) Gel analysis of the integration products. Reactions were performed in the presence (+) or in absence (−) of 250 nM of IN. The target DNA is indicated as pBSK receptor plasmid. The donor DNA was 5′-end labeled. Products were submitted to 1% agarose gel electrophoresis. nCI, CI: respectively non-concerted and concerted full-site integration products. SI: Self-integration products. M: Markers (base pairs). The structure of the expected products are also described in this figure. (B) Effect of reaction conditions on the integration reaction catalyzed by recombinant HIV-1 IN. For each condition one parameter was changed independently (MgCl₂ or MnCl₂ concentration, presence of DMSO or PEG). The percentage of the total integrated substrate was reported on the graph. Results are the mean +/− SD of three separate experiments.