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. 2005 Feb 17;33(3):987–998. doi: 10.1093/nar/gki243

Figure 8.

Figure 8

Gfi1b binds to the Gfi1 and Gfi1b promoters sequences in vivo. (a) Cells from spleen of two wild-type mice were isolated and CH-IP was performed separately using an α-Gfi1b antibody or no antibody as a control. The Gfi1b-promoter region could be amplified from 0.01% of input DNA (lanes 1 and 2) or immunoprecipitated DNA (lanes 3 and 4) only if the Gfi1b antibody was used for immunoprecipitation, but not without antibody (lanes 5 and 6). (b) B220+ cells from spleen of wild-type and vav-Gfi1b transgenic mice were isolated and ChIP was performed using an α-Gfi1b antibody. The Gfi1b-promoter region (Gfi1b locus) and the Gfi1 promoter region (Gfi1 locus) could be amplified from 0.01% of input DNA or immunoprecipitated DNA. For the Gfi1b locus, two representative experiments are shown. A more prominent band is observed if cells from vav-Gfi1b transgenic mice were used for ChIP. As a negative control, primers from the CD95 gene locus were used.