Figure 1.

Aloesin inhibits SKOV3 cell growth in a dose- and time-dependent manner. (a) Five ovarian cancer cells were assayed for cell viability determination with the doses of aloesin treatment ranging from 2.5 μM to 40 μM. Cells with 0 μM aloesin treatment were used as control. All groups of cells were cultured for 48 h. (b) At a fixed dose (5 μM), aloesin inhibited five ovarian cancer cell viabilities in a time-dependent manner (24 h, 48 h, and 72 h). (c) Colony formation assays were performed using SKOV3 cells treated with indicated doses of aloesin. The clones were stained with crystal violet and shown. (d) In the colony formation assay, clones were formed and counted manually for each group of cells. (e) The size of each clone was also calculated and averaged. ^∗p < 0.05; ^∗∗p < 0.01; ^∗∗∗p < 0.001.