Table 1.
Primers and pyrosequencing assay information. Primers sequences used for PCR, Sanger sequencing, pyrosequencing and AFLP technique
| NRAS Pyrosequencing ASSAY 1 | |
| PCR 124 nt | |
| Primer F-Biotine | ACACCCCCAGGATTCTTACAGA |
| Primer R | GCCTGTCCTCATGTATTGGTC |
| Pyrosequencing primer | CATGGCACTGTACTCTTC |
| Nucleotide injection order | GTTACGTCAGCTG |
| NRAS Pyrosequencing ASSAY 2 | |
| PCR 124 nt | |
| Primer F-Biotine | ACACCCCCAGGATTCTTACAGA |
| Primer R | GCCTGTCCTCATGTATTGGTC |
| Pyrosequencing primer | CATGGCACTGTACTCTTC |
| Nucleotide injection order | GCATACGTCAG |
| NRAS Pyrosequencing ASSAY 3 | |
| PCR 90 nt | |
| Primer F-Biotine | ACAAGTGGTTATAGATGGTGA |
| Primer R | ATGTATTGGTCTCTCATGGCA |
| Pyrosequencing primer | CATGGCACTGTACTCTTC |
| Nucleotide injection order | GCATACGTCAGCT |
| Sanger Sequencing | |
| Primer F | ACAAGTGGTTATAGATGGTGA |
| Primer R | ATGTATTGGTCTCTCATGGCA |
| AFLP primers | |
| rs3219599 F | TTCAAGGCTGCAGTGAGCTA |
| rs3219599 R | AGTGGAAGCTAGACACACATTAAGA |
| s3219653 F | CCAGAGAGACAGAACTGAACAAA |
| s3219653 R | CAAATTTTGGACCTGCCATG |
| rs3219587 F | GGGCAAATGGAGGAAAGAGA |
| rs3219587 R | TAAAAATACCCCCACCCCACT |
| rs3220698 F | TTAAAAAACGTACTGCCACATTCA |
| rs3220698 R | GGCAGAAACCAGGAAATGTAGTA |
| rs3220987 F | GGCTTTTAGCTATGATTTGAGA |
| rs3220987 R | GACTCAGGAAATAAACAAGGC |
| rs3220389 F | CGCTGCTCACTCCTCCTCTGA |
| rs3220389 R | AGTGCTGCTCTCAGTGAACTC |
| rs3219612 F | AGCACACAATATACTCTCTCAGA |
| rs3219612 R | ACCTGGGCAAAAGAGTAAGACC |
| rs3219703 F | AACGAAGGTGTACTGGGACTGGT |
| rs3219703 R | ACAGGGATGTGAGGGATTTTTTC |