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. 2017 Jul 1;17:460. doi: 10.1186/s12885-017-3430-2

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© The Author(s). 2017

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 4 — RanBPM requires the LisH and CTLH domains to associate with α-tubulin. Hela RanBPM shRNA cells were transfected with the indicated HA-RanBPM deletion constructs. Cells were fixed and incubated with antibodies against HA (green) and α-tubulin (red). Colocalization of HA-RanBPM and α-tubulin was analyzed using Imaris software. Top, representative images where white signal represents the top 2% of HA-RanBPM and α-tubulin colocalization. Scale bar: 10 μm. Bottom, Quantification of the top 2% of pixels representing HA-RanBPM colocalized with α-tubulin. Data are representative of a minimum of 10 transfected cells from three experiments. Error bars represent SEM. P < 0.05 (*)