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. Author manuscript; available in PMC: 2017 Jul 2.
Published in final edited form as: Biotechniques. 2015 Apr 1;58(4):200–202. doi: 10.2144/000114277

Figure 2. Frequency of chimeric sequences present in libraries constructed with the three different approaches.

Figure 2

Ligation-based library was prepared using the NEBNext Fast DNA Fragmentation & Library Prep Set for Ion Torrent (New England BioLabs); the MuSeek Library Preparation Kit (Thermo Fisher) and the MuPlus method were used to prepare transposon-based libraries. Numbers above bars indicate frequency of chimeric reads per 1,000,000 total reads. Chimeric reads are defined as described in the text.