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Sequencing metrics and data output on the Ion Proton NGS platform of libraries constructed with three different approaches.
| Metrics | Library preparation protocol | ||
|---|---|---|---|
| Ligation-based | MuSeek | MuPlus | |
| ISP density (%) | 84 | 82 | 78 |
| Polyclonal (%) | 31 | 51 | 23 |
| Low quality (%) | 9 | 55 | 6 |
| Usable reads (%) | 63 | 22 | 72 |
| Reads (n) | 77,160,045 | 26,265,788 | 81,970,145 |
Representative results out of 24 MuSeek-based and 15 MuPlus-based sequencing runs (with 6–9 libraries multiplexed per run) are shown.
