Table 2.
Spontaneous intrachromosomal recombinant frequencies for wild-type and mutant cells
| Strain | Total Ade+ | Deletion-types | Conversion-types | |||||||
|---|---|---|---|---|---|---|---|---|---|---|
| Frequency | Rel. freq.a | Pa | Frequency | Rel. freq.a | Pa | Frequency | Rel. freq.a | Pa | % | |
| Wild-type (FO665) | 3.1 ± 0.7 | 1.0 | — | 2.2 ± 0.5 | 1.0 | — | 1.0 ± 0.2 | 1.0 | — | 31 ± 5 |
| mag1 (FO755) | 10.2 ± 1.8 | 3.3 | ***b | 8.4 ± 1.5 | 3.9 | ***b | 1.8 ± 0.4 | 1.9 | ***b | 18 ± 3 |
| nth1 (FO751) | 20.5 ± 6.3 | 6.6 | ***b | 9.9 ± 2.7 | 4.6 | ***b | 10.5 ± 4.1 | 11 | ***b | 51 ± 5 |
| mag1 nth1 (FO830) | 15.7 ± 3.5 | 5.1 | >0.05c***d | 10.5 ± 2.3 | 4.9 | >0.05c***d | 5.2 ± 1.4 | 5.5 | ***c***d | 33 ± 4 |
| rad16 (FO181) | 21.4 ± 3.5 | 6.9 | ***b | 16.0 ± 2.9 | 7.5 | ***b | 5.3 ± 1.0 | 5.6 | ***b | 25 ± 3 |
| mag1 rad16 (FO839) | 13.0 ± 2.0 | 4.2 | ***e***f | 10.1 ± 1.6 | 4.7 | **e***f | 2.8 ± 0.6 | 3.0 | ***e***f | 22 ± 3 |
| rad2 (FO487) | 68 ± 12 | 22 | ***b | 57.8 ± 9.9 | 27 | ***b | 10.1 ± 2.7 | 11 | ***b | 15 ± 3 |
| mag1 rad2 (FO761) | 43 ± 9 | 14 | ***g***h | 28.9 ± 8.6 | 13 | ***g***h | 14.1 ± 3.0 | 15 | ***g***h | 33 ± 8 |
| rhp55 (FO679) | 10.2 ± 3.3 | 3.3 | ***b | 10.0 ± 3.2 | 4.7 | ***b | 0.12 ± 0.08 | 0.1 | ***b | 1.1 ± 0.8 |
| mag1 rhp55 (FO795) | 19.0 ± 3.8 | 6.1 | ***i***j | 18.8 ± 3.8 | 8.8 | ***i***j | 0.17 ± 0.09 | 0.2 | >0.05i***j | 0.9 ± 0.5 |
Recombinant frequencies (per 104 viable cells ± SD) were obtained as described in Materials and Methods and are the means from 15 independent colonies (three independent assays, five colonies used per assay). The percentage conversion-types (% ± SD) were determined in the same way. ‘*’ indicates a significant difference at the 95% confidence level (0.01 < P < 0.05); ‘**’ indicates a significant difference at the 99% confidence level (0.001 < P < 0.01); and ‘***’ indicates a significant difference at the 99.9% confidence level (P < 0.001).
aRelative frequency (Rel. freq.) is the recombinant frequency of the mutant strain relative to that of the corresponding frequency of the wild-type strain. P-values are from two-sample t-tests in which individual recombinant frequencies and percentage conversion-types from all colonies assayed (15 per strain) were analysed. They were obtained to determine the statistical significance of differences in recombinant frequencies (and percentage of conversion-types) for.
bA given single mutant strain compared with the wild-type strain.
cThe mag1 nth1 double mutant compared with the nth1 single mutant.
dThe mag1 nth1 double mutant compared with the mag1 single mutant.
eThe mag1 rad16 double mutant compared with the rad16 single mutant.
fThe mag1 rad16 double mutant compared with the mag1 single mutant.
gThe mag1 rad2 double mutant compared with the rad2 single mutant.
hThe mag1 rad2 double mutant compared with the mag1 single mutant.
iThe mag1 rhp55 double mutant compared with the rhp55 single mutant.
jThe mag1 rhp55 double mutant compared with the mag1 single mutant. P-values > 0.05 indicates no significant difference between the two strains being compared at the 95% confidence level.