Table 2. Structural statistics for the hRpn13 Pru-hRpn2 complex.
| hRpn13 Pru (20–130) | hRpn2 (940–953) | |
|---|---|---|
| NMR distance and dihedral constraints | ||
| Distance restraints | ||
| Total NOE | 1,641 | 141 |
| Intra-residue | 550 | 75 |
| Inter-residue | 1,091 | 66 |
| Sequential (|i–j|=1) | 438 | 47 |
| Nonsequential (|i–j|>1) | 653 | 19 |
| Hydrogen bonds | 45 | 0 |
| Intermolecular NOEs | 140 | |
| Total dihedral angle restraints | ||
| phi | 161 | 18 |
| psi | 161 | 18 |
| Structure statistics | ||
| Violations (mean and s.d.) | ||
| Distance constraints (Å) | 0.041 | |
| Dihedral angle constraints (°) | 0.390 | |
| Max. dihedral angle violation (°) | 0 | |
| Max. distance constraint violation (Å) | 0 | |
| Deviations from idealized geometry | ||
| Bond lengths (Å) | 0.003±0.000 | |
| Bond angles (°) | 0.454±0.024 | |
| Impropers (°) | 0.330±0.016 | |
| Average pairwise root mean square deviation (r.m.s.d.)* (Å) | ||
| Heavy | 1.55±0.24 | |
| Backbone | 0.81±0.17 | |
*Statistics for secondary structural elements of 12 lowest energy with best geometry structures within hRpn13 Pru (V24-K34, T37-P40, G45-Q51, I57-D63, N68-I75, E81-K83, Y94-K97, R104-W108 and D117-L128) and hRpn2 P942-I951.