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. 2005 Feb 7;33(3):e24. doi: 10.1093/nar/gni014

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© The Author 2005. Published by Oxford University Press. All rights reserved

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Mini-Mu transposons for the construction of targeting vectors. Each transposon has one or two antibiotic resistance genes flanked by transposon ends (filled black squares) with LoxP (open triangle) and/or restriction sites. PGK, the human PGK promoter; Cam^r, bacterial chloramphenicol resistance gene; Tet^r, bacterial tetracycline resistance gene; Neo^r, G-418 resistance gene without a promoter; PGK-Neo^r, G-418 resistance gene driven by the PGK promoter; P/P-Kan^r/Neo^r, eukaryotic/prokaryotic dual promoters driving the expression of kanamycin resistance in E.coli and G-418 resistance in mammalian cells.