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Schematic representation of generating targeting vectors using TnCR1 and TnCR2. TnCR1 is inserted into the target gene by in vitro transposition. TnCR2 is then inserted at the other side of the region to be deleted. Deletions are generated between the two LoxP sites by one of two Cre treatments. First, DNA is electroporated into an E.coli strain expressing the Cre recombinase. Second, the construct is treated with Cre recombinase (Invitrogen) and then transformed to E.coli.
