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. 2005 Feb 7;33(3):e24. doi: 10.1093/nar/gni014

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© The Author 2005. Published by Oxford University Press. All rights reserved

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Schematic representation of generating targeting vectors using TnCR3 and TnCR4. TnCR3 is inserted into the target gene by in vitro transposition. TnCR4 is then inserted at the other side of the region to be deleted. Deletions are generated by digesting the DNA with one of the restriction endonucleases followed by ligation and transformation to E.coli.