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. 2005 Feb 7;33(3):e24. doi: 10.1093/nar/gni014

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© The Author 2005. Published by Oxford University Press. All rights reserved

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Schematic representation of generating conditional targeting vectors using TnCR7 and TnCR8. TnCR7 is inserted into the target gene by in vitro transposition. The Cam^r gene is deleted using either Cre or a restriction endonuclease, leaving a single copy of LoxP. TnCR8 is then inserted at the other side of the region to be deleted. The construct is now a conditional targeting vector.