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miR-150 Suppresses Expression of Glut1. (A) Sequences of miR-150 and the potential binding sites at the 3’UTR of Glut1. (B) Western blot assays show that overexpression of miR-150 suppresses the expression of Glut1. (C) Real time PCR show that overexpression of miR-150 increases Glut1 mRNA level. (D) Dual-luciferase assays show that miR-150 suppresses the luciferase activity in Glut1-3’UTR wild type group, but don’t in Glut1-3’UTR mutant group.
