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. 2005 Feb 18;33(3):e30. doi: 10.1093/nar/gni026

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© The Author 2005. Published by Oxford University Press. All rights reserved

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The excerpt is from our analysis of the published data. The percentage mRNA remaining is from Vickers et al. (33). As mentioned, effective sites are those which result in 55% or greater knockdown. For duplex energies, sites where the 5′ antisense end is 0.5 kcal/mol or more stable than the 3′ end are deemed unfavorable. The acceptable range for GC-content is 30–52%. And sites predicted to be accessible are those with a predicted relative accessibility of 0.55 or higher. The superscript letter ‘a’ represents the columns from Vickers et al. (33).