Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2005 Feb 17;33(3):e29. doi: 10.1093/nar/gni029

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author 2005. Published by Oxford University Press. All rights reserved

The online version of this article has been published under an open access model. Users are entitled to use, reproduce, disseminate, or display the open access version of this article for non-commercial purposes provided that: the original authorship is properly and fully attributed; the Journal and Oxford University Press are attributed as the original place of publication with the correct citation details given; if an article is subsequently reproduced or disseminated not in its entirety but only in part or as a derivative work this must be clearly indicated. For commercial re-use, please contact journals.permissions@oupjournals.org

PMC Copyright notice

Scatter plots of log₂-transformed expression ratios (log₂ Cy5/Cy3) from duplicate hybridizations. Amplified breast and reference RNA, with and without reversed assignment of fluorophores (dye swap) was employed to evaluate the accuracy and reproducibility of the experiment. Replicate spots were averaged. (A) 2000 ng; (B) 2000 ng, dye swap; (C) 200 ng; (D) 200 ng, dye swap; (E) 20 ng; (F) 20 ng, dye swap; (G) 2 ng; (H) 2 ng, dye swap. The data were subjected to orthogonal regression analysis (red lines), and associated linear equations are listed along with Pearson correlation coefficients. The 95% confidence intervals of the correlation coefficients are (0.9946, 0.9950), (−0.9809, −0.9796), (0.9885, 0.9892), (−0.9513, −0.9478), (0.9772, 0.9788), (−0.9444, −0.9404), (0.9936, 0.9940) and (−0.9050, −0.8983) for (A) through (H). Underlying microarray images are shown as fixed sections in an upper (ordinate) and lower (abscissa) corner of each plot.

Scatter plots of log₂-transformed expression ratios (log₂ Cy5/Cy3) from duplicate hybridizations. Amplified breast and reference RNA, with and without reversed assignment of fluorophores (dye swap) was employed to evaluate the accuracy and reproducibility of the experiment. Replicate spots were averaged. (A) 2000 ng; (B) 2000 ng, dye swap; (C) 200 ng; (D) 200 ng, dye swap; (E) 20 ng; (F) 20 ng, dye swap; (G) 2 ng; (H) 2 ng, dye swap. The data were subjected to orthogonal regression analysis (red lines), and associated linear equations are listed along with Pearson correlation coefficients. The 95% confidence intervals of the correlation coefficients are (0.9946, 0.9950), (−0.9809, −0.9796), (0.9885, 0.9892), (−0.9513, −0.9478), (0.9772, 0.9788), (−0.9444, −0.9404), (0.9936, 0.9940) and (−0.9050, −0.8983) for (A) through (H). Underlying microarray images are shown as fixed sections in an upper (ordinate) and lower (abscissa) corner of each plot.