Figure 2.

Processed screening data for zinc finger libraries. Randomized protein libraries 1–60 were screened against target DNA sequences containing the triplets (A) TCC, (B) GCA, (C) GTT, (D) ACG and (E) TAC. The resulting data were scaled according to the relative GFP fluorescence in each protein library, normalized (where 100% = the highest signal after three washes) and sorted by the highest signal. Numbers on the ordinate are normalized DNA binding (%) and letters on the abscissa are the identity of the fixed amino acid in positions −1, 3 and 6 (Figure 1). Filled bars represent data measured after three washes and open bars represent data obtained after four washes, as described in Materials and Methods. All experiments were performed in triplicate. Error bars represent 1 SD.