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. 2017 Jul 3;3:17025. doi: 10.1038/cddiscovery.2017.25

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Copyright © 2017 The Author(s)

This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/

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SFN-Cys-induced dose-dependent apoptosis in human A549 and SK-1 cells. (a) A549 and (b) SK-1 cells were treated with various concentrations of SFN-Cys (0, 10, 20 and 30 μM) for 24 h, and stained with Annexin V-FITC/PI. Compared with the control cells, flow cytometry revealed that the apoptotic rates of the SFN-Cys treated (c) A549 and (d) SK-1 cells were significantly increased (P<0.01), whereas the apoptotic rates of the SFN-Cys-treated cells combined with PD98059 was declined (P<0.05). These results represented three independent tests. Error bars indicate uncertainty errors in graphs to relay 95% of confidence in the interpreted data. * Indicates P<0.05 versus the control group. ** Indicates P<0.01 versus control group. ^# Indicates P<0.05 versus the SFN-Cys-only group.