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Surface area calculations of the branch site RNA helices are as described in the Methods
| Total SASA (Å2) | Polar/nonpolar SASA | Nonpolar SASA of branch site adenosine (Å2) | SASA of 2′-OH branch site adenosine (Å2) | |
|---|---|---|---|---|
| ψψBP | 3770 ± 168 | 1.95 | 110 ± 30 | 0.4 ± 1.8 |
| uBP | 3670 ± 39 | 1.9 | 56 ± 6 | 18 ± 3.9 |
The calculations are averaged over the ensembles of all NMR models for both modified and unmodified RNA structures. Values were calculated by NACCESS using the radii published by Alden and Kim (16).
