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. 2017 May 22;36(13):1928–1945. doi: 10.15252/embj.201695622

Figure EV4. Numb segregation and Prospero segregation.

Figure EV4

  • A, B
    (A–A′′) Prospero was absent in most divisions (94.6%) showing an asymmetric distribution of Numb‐GFP. (B–B′′) However in some divisions (5.4%), co‐localization with Numb‐GFP was observed (n = 74; Numb‐GFP, green in A and B, white in A′ and B′; Pros, red in A and B, white in A′′ and B′′; PH3, white; DAPI, blue). Scale bar = 2 μm.
  • C
    Upon expression of esgGal4, tub‐Gal80ts‐driven UAS‐ttk69‐RNAi, Numb‐GFP showed an asymmetric distribution in 77.7% of late anaphases (n = 9; PH3, red; Numb‐GFP, green in C, white in C′; DAPI, blue).
  • D
    Upon expression of esgGal4, tub‐Gal80ts‐driven UAS‐aPKC‐CAAX, Numb‐GFP showed symmetric distribution in 92.9% of late anaphases (n = 17; PH3, red; Numb‐GFP, green in D, white in D′; DAPI, blue).
  • E
    In lgl 4 mutant clones, Numb‐GFP showed asymmetric distribution in 100% of late anaphases (n = 4; PH3, red; Numb‐GFP, green in E, white in E′; DAPI, blue).