Figure 6. Numb controls EE fate through Notch signaling inhibition.

• A–K
  (A–B′; F–G′) 10 days AHS MARCM clones outlined with dotted lines (GFP, green; EE cells marked by Pros, in red or white as indicated; DAPI, blue). (A, A′) Notch RNAi resulted in extra EE cells. (B, B′) Similarly, a numb ¹⁵ mutant clone expressing Notch RNAi contained extra EE cells. Quantification of: (C) clone size, (D) the average number of EE cells per clone, and (E) the percentage of EE cells per clone. (F, F′) EE cells were greatly reduced in ada ^ear4 mutant clones. (G, G′) Expression of UAS‐numb did not promote EE cell production in ada ^ear4 mutant clones. Quantification of: (H) clone size, (I) the average number of EE cells per clone, (J) the percentage of EE cells per clone, and (K) and percentage of clones containing at least one EE cell.

Data information: Scale bars = 30 μm. Results were compared using two‐tailed Mann–Whitney statistical test for (C, D) and the Fisher's exact test for (E) (P‐values indicated). The bars represent mean values ± SEM.