Figure 7. Numb is an upstream regulator of EE fate determination.

• A–K
  (A–F′, J–K′) 10 days AHS MARCM clones (GFP, in green) outlined with dotted lines; Pros, red or white as indicated or otherwise specified; DAPI, blue). (A, A′) EE cells were formed upon UAS‐scute expression and (B, B′) when UAS‐scute was expressed in numb ¹⁵ mutant clones. (C, C′) Similarly, UAS‐asense promoted EE cell formation in wild‐type or (D, D′) in UAS‐asense, numb ¹⁵ mutant clones. (E, E′) Ttk69 RNAi produced ectopic EE cells alone as well as (F, F′) in combination with numb ¹⁵ mutant. Quantification of (G) cells per clone, (H) average number of EE cells per clone, and (I) percentage of EE cells per clone. EE cells (marked by Tk in red or white) were lost in pros ¹⁷ mutant clones, (J, J′) The expression of UAS‐numb failed to promote EE cells in pros ¹⁷ mutant clones (Tk, red or white in F′). (K, K′) Extra EE cells were formed upon ttk69 RNAi expression. (F, F′) Likewise, ttk69 RNAi expression promoted EE fate in numb ¹⁵ mutant clones.

Data information: (G–I) Results were compared using a two‐tailed Mann–Whitney statistical test (ns = non‐significant, P‐values indicated). Scale bar = 30 μm. The bars represent mean values ± SEM.