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. 2017 Jan 26;30(8):1124–1134. doi: 10.5713/ajas.16.0677

Table 4.

Effect of heat treatment and serial dilution on chemical compositions (electrolytes and metabolites) of different concentrations of HPA solutions

Concentration of HPA (mg/mL) Heat treatment (minutes) Ca2+ (mmol/L) K+ (mmol/L) Glucose (mmol/L) Lactate (mmol/L) Acid-base status (mmol/L)
150.0 0 0.27 ND 0.25c 1.65a −34.1i
30 0.35 ND 0.50bc 1.25b −34.2j
60 0.45 ND 0.75ab 1.40ab −34.0h
75.0 0 0.32 ND 0.50bc 0.50e −33.7g
30 0.36 ND 0.75ab 0.85cd −34.0h
60 0.41 ND 0.70b 0.65de −33.7g
37.5 0 0.33 19.85 0.55bc 0.50e −33.3e
30 0.38 18.75 0.70b 0.60de −33.7g
60 0.39 18.95 1.10a 1.10bc 33.4f
18.75 0 0.28 9.95 0.30c ND −32.7c
30 0.40 9.85 0.25c ND −33.0d
60 0.38 9.85 0.40bc ND −33.0d
9.375 0 0.25 5.10 ND ND −32.0a
30 0.34 4.95 ND ND −32.3b
60 0.34 5.00 ND ND −32.3b
SEM 0.350 1.430 0.050 0.100 0.13
Main effects
 150.0 0.36a ND 0.50c 1.43a −34.1e
 75.0 0.36a ND 0.65b 0.67b −33.8d
 37.5 0.36a 19.18a 0.78a 0.73b −33.5c
 18.75 0.35a 9.90b 0.32d ND −33.0b
 9.375 0.31b 5.02c ND ND −32.2a
0 0.29b 11.63a 0.40c 0.88 −33.2a
30 0.36a 11.18b 0.60b 0.90 −33.4c
60 0.39a 11.27b 0.74a 1.05 −33.3b
p-values
 Concentration of HPA <0.05 <0.001 <0.001 <0.001 <0.001
 Heat treatment <0.001 <0.05 <0.001 0.159 <0.001
 Concentration of HPA×heat treatment 0.178 0.111 <0.05 <0.01 <0.001

HPA, Hamlet Protein AviStart; ND, not detected; SEM, standard error of mean.

a–j

Mean values in same column not sharing a superscript are significantly different (p<0.05).