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The gRNA expression vector and overview of the experiment. (A) Expression vector and Cas9-green fluorescent protein (GFP) and qSH3RF2 gRNA sequences. The U6 promoter controls gRNA transcription followed by a termination signal. (B) Experimental overview. After transfection and sorting, single cell-derived knockout sublines were established and the genotypes were subsequently identified by cloning and sequencing.
