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. 2016 Dec 27;30(8):1183–1189. doi: 10.5713/ajas.16.0865

Figure 3.

Figure 3

Genomic mutation analysis in single cell-derived knockout quail myoblast (QM7) cells. (A) T7E1 assay for the targeted sites in quail SH3RF2 in knockout (KO) QM7 cells. (B) Mutated target DNA sequences of SH3RF2 KO QM7#1 and #4 cells. The dashed and red sequences denote the deleted nucleotides and targeted sequences, respectively. SH3RF2 KO QM7#4 cells exhibited the mutant genotype (61 and 155 nucleotide deletions), whereas SH3RF2 KO QM7#1 cells exhibited a mixed genotype (15, 33, and 70 nucleotide deletions).