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. 2017 Jun 6;18(7):1166–1185. doi: 10.15252/embr.201643686

Figure 4. HDAC4 cytoplasmic localization is controlled by the activity of salt‐inducible kinase (SIK).

Figure 4

  1. Representative images of VSMCs transfected with wild‐type GFP‐HDAC4 and treated with the indicated inhibitor and concentration: LMB, leptomycin B; PKDi, CID 2011756; CaMKi, KN‐93; SIKi, HG‐9‐91‐01. DAPI staining was used to mark the nucleus. The nuclear export inhibitor leptomycin B was used as a positive control and induced nuclear accumulation of GFP‐HDAC4. The pan‐SIK inhibitor induced dose‐dependent nuclear accumulation of GFP‐HDAC4. Scale bar = 10 μm.
  2. HDAC4 localization was scored automatically as being exclusively cytoplasmic (white), exclusively nuclear (light gray) or as occupying both a cytoplasmic and nuclear localization (dark gray). Data are shown as means ± SD (n = 250 cells at least in each group). Chi‐square proportion test, *P < 0.001 vs. negative control.