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. 2017 May 24;9(7):950–966. doi: 10.15252/emmm.201607265

Figure 1. The processive γ‐secretase cleavage pattern of multiple substrates.

Figure 1

  1. Substrates based on Aβ peptide and γ‐secretase substrate TMDs for cell‐free assay.
  2. Western blot of substrates overexpressed and purified from BL21.
  3. Aβ‐Nβ chimeric peptides (NTF) IP‐MS of recombinant substrates after incubation with CHAPSO‐solubilized CHO cell membrane with (red) or without (black) 1 μM LY411575.
  4. COOH‐terminal fragments (CTFs) IP‐MS of recombinant substrates with (red) or without (black) 1 μM LY411575.