Table 1.
Molecular weight of Aβ‐chimeric peptides and intracellular domain detected in cell‐free assay and cell‐based assay
| Cell‐free assay | Cell‐based assay | ||||
|---|---|---|---|---|---|
| Peptide | Observed m/z | Calculated MW | Observed m/z | Calculated MW | |
| APPC100sub | CTF_L720a | 7016.8 | 7018.8 | – | – |
| CTF_V721 | 6903.5 | 6905.7 | – | – | |
| A713b (Aβ42) | 4644.1 | 4645.3 | 4671.2 | 4670.2 | |
| V711 (Aβ40) | 4460.2 | 4461.1 | 4486.2 | 4486.0 | |
| G709 (Aβ38) | 4261.5 | 4262.8 | 4288.0 | 4287.7 | |
| G708 (Aβ37) | 4204.8 | 4205.7 | 4230.1 | 4230.7 | |
| NOTCH1sub | CTF_V1754 | 8586.7 | 8588.5 | – | – |
| F1748 | 5014.6 | 5013.6 | – | – | |
| V1745 | 4639.9 | 4640.1 | 5772.6 | 5771.4 | |
| F1744 | 4540.9 | 4541.0 | 5672.9 | 5672.3 | |
| A1743 | 4392.2 | 4393.8 | 5526.4 | 5525.1 | |
| A1741 | 4251.9 | 4251.7 | 5383.2 | 5382.9 | |
| A1740 | 4179.5 | 4180.6 | 5313.1 | 5311.8 | |
| V1739 | – | – | 5240.3 | 5240.8 | |
| Y1738 | 4009.4 | 4010.4 | – | – | |
| M1737 | 3847.7 | 3847.2 | – | – | |
| H1735 | 3567.6 | 3568.8 | – | – | |
| L1734 | 3430.2 | 3431.7 | – | – | |
| NOTCH2sub | CTF_V1697 | 8573.2 | 8571.5 | – | – |
| V1687 | 4341.0 | 4340.9 | 5473.5 | 5472.2 | |
| A1685 | 4142.1 | 4142.6 | 5274.5 | 5273.9 | |
| V1684 | 4074.4c | 4071.5 | 5203.3 | 5202.8 | |
| A1683 | 3972.0 | 3972.4 | 5103.9 | 5103.7 | |
| L1682 | 3900.2 | 3901.3 | – | – | |
| L1681 | 3788.1 | 3788.2 | – | – | |
| L1679 | 3511.7 | 3511.8 | – | – | |
| NOTCH3sub | CTF_G1661 | 8452.5 | 8449.7 | – | – |
| CTF_V1662 | 8395.4 | 8395.3 | – | – | |
| A1651 | 4289.9 | 4290.8 | – | – | |
| G1650 | 4219.1 | 4219.7 | – | – | |
| A1649 | 4161.0 | 4162.7 | 5295.8 | 5294.0 | |
| L1647 | 3991.7 | 3992.5 | – | – | |
| L1646 | 3878.5 | 3879.3 | – | – | |
| P1645 | 3765.4 | 3766.2 | 4898.0 | 4897.5 | |
| NOTCH4sub | CTF_V1467 | 8357.5 | 8356.5 | – | – |
| L1459 | 4683.9 | 4683.3 | – | – | |
| I1458 | 4570.1 | 4569.2 | – | – | |
| G1456 | 4358.1 | 4357.8 | – | – | |
| CD44sub | A659 | 4548.5 | 4545.1 | – | – |
| L658 | 4475.9 | 4474.0 | – | – | |
| L657 | 4361.4 | 4360.9 | – | – | |
| S656 | 4248.5 | 4247.7 | – | – | |
| A655 | 4159.7 | 4160.6 | – | – | |
| L654 | 4090.4 | 4089.6 | – | – | |
| VEGFR1sub | CTF_T777 | 8543.8 | 8545.7 | – | – |
| T765 | 4399.0 | 4398.8 | – | – | |
| T763 | 4195.6 | 4194.6 | – | – | |
a
NH2‐terminal amino acid residue.
b
COOH‐terminal amino acid residue.
c
Overlapped with a non‐specific peak cause the value shift ~4 Da.