Figure EV2. Cardiomyocyte quantification and characterization by FACS and qRT–PCR.

1. FACS staining for cTNNT2 in D10 and D15 human ES cell‐derived cardiac cells. IgG₁ isotype was used as a control.
2. POU5F1 mRNA expression levels in human ES cells and D15 as well as D30 cardiac cells, as measured by qRT–PCR. Expression levels were normalized to a non‐oscillatory housekeeping gene (PPIA). Data are represented as mean ± s.e.m. of three independent replicates.