Figure 1. DHX9 is a nucleolar protein that associates with IGS‐rRNA and TIP5.

1. Schema represents the GRNA chromatography employed for the identification of IGS‐rRNA‐binding proteins.
2. Representative mass spectrometry analysis of proteins pulled down with IGS‐rRNA and found enriched in all three independent experiments (labeled with orange circles), using nuclear extracts from NIH 3T3 cells or NPCs. Values (green circles) refer to peptide numbers obtained in each experiment. For direct comparison with the other experiments, the same data are shown in Fig EV1B.
3. DHX9 localizes in nucleoli. Immunofluorescence analysis with DHX9 antibodies in U2OS cells and with GFP antibodies in NIH 3T3 cells transfected with GFP‐DHX9 plasmids. Nucleoli are visualized with anti‐UBF.
4. Endogenous DHX9 associates with TIP5. Schema represents the domain organization of TIP5. Anti‐FLAG immunoprecipitation from HEK293T cells expressing FLAG‐HA‐TIP5 or FLAG‐HA‐TIP5_1–700. Immunoblots show association of TIP5 with DHX9 but not with the RNA Pol II.

Source data are available online for this figure.