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. 2017 Apr 13;9(7):859–868. doi: 10.15252/emmm.201607486

Figure 1. Validation of a novel poly‐GP‐specific immunoassay.

Figure 1

  • A
    Immunohistochemistry of frontal cortex from ALS/FTD cases with or without C9orf72 repeat expansion using poly‐GP antibodies 18H8 and 3F9. Both antibodies detect neuronal cytoplasmic inclusions specifically in the C9orf72 case (arrows). Hybridoma supernatants were used at 1:250 dilution as described previously (Schludi et al, 2015). Scale bar 20 μm.
  • B, C
    Poly‐GP sandwich immunoassay with anti‐GP antibodies 18H8 and 3F9 detects purified GST‐GP15 below 0.03 ng/ml (B), but no other 15‐mer DPRs fused to GST at 1 μg/ml. Data are shown as mean ± SD (n = 2) (C). A four‐parameter logistic curve was used to fit the dose–response using Prism 7.01 software.