Fig. 4.

Central TCR accumulation associates with sustained actin accumulation but not with critical effector functions in P14 and HY T cells. (A and B) Central TCR accumulation and effector functions in primary T cell/EL4 APC interactions (P14 T cells in A and HY T cells in B) under the indicated agonist peptide concentrations are shown as normalized data similar to Fig. 1. Raw data are given in Table 1 and Fig. 2. (C) The percent of actin-GFP-transduced P14/EL4 cell couples showing actin accumulation at the T cell/APC interface at >40% of the cellular background fluorescence intensity at the indicated time after cell couple formation in the presence of the given gp33 agonist peptide concentrations are displayed. Differences between values at 10 μM versus 1 μM and 100 nM gp33 peptide at 4′ and 5′ are significant (P ≤ 0.001). Between 22 and 36 cell couples from at least two independent experiments were analyzed per condition.