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. 2017 Mar 29;312(6):C733–C740. doi: 10.1152/ajpcell.00021.2017

Fig. 4.

Fig. 4.

Albumin inhibits endocytosis of hemoglobin by OK cells. A: OK cells were incubated with apically added 2 µM Alexa Fluor 568-Hb in the presence or absence of 50 µM unlabeled OxyHb or 30 µM albumin for 1 h at 37°C, then fixed and processed for immunofluorescence. As a control to confirm that OxyHb fluorescence is not detected in our studies, cells were incubated for 1 h with 50 µM unlabeled OxyHb (right-hand panel) Scale bar, 25 µm. B: OK cells were incubated with apically added 2 µM Alexa Fluor 568-Hb in the presence or absence of the incubated concentrations of albumin or unlabeled Hb for 1 h at 37°C prior to quantitation of cell-associated Hb fluorescence. Data from two experiments performed in triplicate are shown. Background fluorescence in cells incubated only with 50 µM unlabeled OxyHb was <10% that of the control values.