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. 2017 Mar 29;312(6):C724–C732. doi: 10.1152/ajpcell.00348.2016

Fig. 2.

Fig. 2.

Autophagy-related protein content during adult skeletal muscle regeneration. A: representative histological images of regenerating muscle at 1, 3, and 14 days postinjury relative to contralateral uninjured control: scale bar = 200 μm. B: representative immunoblot images of Ulk1, Beclin1, p62, LC3-I, LC3-II, myogenin, Cyt C, COX4, GAPDH, and 14-3-3- for cardiotoxin (Ctx)-injured muscles and contralateral control muscles at 1, 3, 7, and 14 days postinjury (D1, D3, D7, and D14). C: quantification of Beclin1, Ulk1, and total LC3, collectively an index of autophagy capacity, during muscle regeneration. D: quantification of p62, LC3-II, and LC3-II/I ratio collectively an index of autophagy flux, during muscle regeneration. E: quantification of COX4 and Cyt C as a measure of mitochondrial content. Results are represented as means ± SE from n = 5 mice for each day. *P < 0.05, **P < 0.01, ***P < 0.001, significantly different from contralateral control.